Diazotization of catecholamines and their analogs and metabolites for urinary screening tests: chemical aspects.

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Diazotization of catecholamines and their analogs and metabolites for urinary screening tests: chemical aspects.

Coupling of diazotized p-nitroaniline to catecholamines and their metabolites in urine has been proposed for use in screening for secreting neuroblastoma in childhood. We have coupled diazotized p-nitroaniline to catecholamines, derivatives, and metabolites and examined the reaction products by thin-layer chromatography and physico-chemical methods (ultraviolet spectra, mass spectroscopy, nucle...

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Chemical basis and specificity of chemical screening tests for urinary vanilmandelic acid.

The most common metabolite of neural crest tumors is vanilmandelic acid. There have recently been several screening tests for this substance in urine, in which diazotized aromatic amines couple with it to produce colored azo dyes. The general opinion has been that these tests are very nonspecific, yet little has been written concerning the exact chemical basis of the reaction with respect to bi...

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The effects of illness on urinary catecholamines and their metabolites in dogs.

BACKGROUND Urinary catecholamines and metanephrines have been proposed as a diagnostic tool for identifying canine pheochromocytomas, but the effects of critical illness on urine concentrations of catecholamines and metanephrines currently are unknown. OBJECTIVES To examine the effects of illness on urine concentrations of catecholamines and metanephrines in dogs. ANIMALS Twenty-five critic...

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Effects of Trilostane on urinary Catecholamines and their metabolites in dogs with Hypercortisolism

BACKGROUND Glucocorticoids influence the synthesis and metabolism of catecholamines (epinephrine and norepinephrine) and metanephrines (metanephrine and normetanephrine). The aim of this study was to measure urinary catecholamines and metanephrines in dogs with hypercortisolism before and during trilostane therapy. Urine samples were collected during initial work up and during therapy with tril...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1976

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/22.12.1955